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. 2014 Jul 23;15:30. doi: 10.1186/1471-2121-15-30

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Copyright © 2014 Ngo et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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MXD3 activation induces a transient increase in BrdU incorporation. Bromodeoxyuridine proliferation assay of ER-MXD3.E66D and ER-MXD3 cell lines treated with 1 μM 4-OHT normalized to vehicle. Treatments were staggered such that all conditions were assayed at the same time for BrdU incorporation after a 2 hour BrdU pulse. ER-MXD3 significantly (p = 0.0487, two-way ANOVA, n = 8) responds to 4-OHT treatment compared to vehicle, whereas ER-MXD3.E66D does not. Results of bonferroni post-test analysis comparing 4-OHT to vehicle are noted with asterisks.