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. 2014 Jul 7;14:224. doi: 10.1186/1472-6882-14-224

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Copyright © 2014 Wu et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Gene expressions and collagen production in NIH/3T3 fibroblasts with or without TGF-β1 and/or Yiqihuoxue treatment. (A) Relative transcript levels of Col1a2, Col3a1, Ctgf, Sparc and Tgf-β1 in murine 3T3 fibroblasts with different treatments. N, non-treated; T, TGF-β1; Y, Yiqihuoxue decoction. The expression level of each gene in non-treated murine 3T3 fibroblasts was normalized to 1. *, P <0.05; **, P <0.001. (B) Western blotting analysis of type I collagen (COL1) in murine 3T3 fibroblasts with different treatments. (C) Densitometric analysis of western blotting for type I collagen (COL1). Bars showed the mean ± SD results of three assays. *, P <0.05; **, P <0.001.