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. 2014 Jul 11;14:386. doi: 10.1186/1471-2334-14-386

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Copyright © 2014 Tatro et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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HIV p24 measured in supernatant. Microglia were exposed for 4 hr to stock HIV_BaL virus (equivalent to 5,000 pg/mL of p24), then washed in PBS. Aliquots of extracellular supernatant were then removed immediately (day 1 on plot) and after 9 d in culture, then p24 measured by ELISA. Plotted are means of three biological replicates with standard deviation. ND — not detected. P24 was was significantly higher in HIV + than HIV + RAL at 9 d (P < 0.001), and HIV + RAL was not significantly different from uninfected at 9 d (P = 0.19), by ANOVA and Tukey Honestly Significant Difference test.