Figure 4.

Effects of baicalein (BE) on lysoPC-induced apoptosis. H9c2 cells were pretreated with or without BE before being stimulated by lysoPC (10 μM). (a) Fluorescence photomicrographs of cells stained with Hoechst 33342, showing nucleus exhibiting brightly stained condensed chromatin, indicating typical morphological changes of apoptosis. (b) Analysis of apoptotic cells defined by annexin V-positive cells. The effects of BE (1, 5, and 10 μM) on lysoPC-induced apoptosis of H9c2 cells were determined by flow cytometry. Quantitative bar graphs showing apoptotic cell counts determined by flow cytometry. Each value represents the mean ± SEM of three independent experiments, with triplicate determinations in each experiment. ^#P < 0.05 compared with control; **P < 0.01 versus cells exposed to lysoPC alone.