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. 2014 Jul 1;11:50. doi: 10.1186/1742-4690-11-50

Figure 1.

Figure 1

Cyclin T1 point mutations screened for Hexim1 binding deficiency by yeast two-hybrid. (A) Distribution of Cyclin T1 single mutations generated by random mutagenesis. Colours mark the sequences that were amplified in four different error-prone PCR reactions: I – from residue 1 to 106 (yellow), II – from 88 to 188 (green), III – from 88 to 261 (cyan) and IV – from 140 to 261 (magenta). The position of single amino-acid mutations obtained following each PCR are highlighted in black. Several mutations are found outside the PCR amplified sequence. (B) List of Cyclin T1 point mutations obtained by “Random” or “Targeted” mutagenesis. Numbers in brackets indicate the mutations that occurred more than once. Wild-type interaction (+++), decreased interaction (+) and lack of interaction (−) are deduced from regular, slow or no growth on LTU selective medium, respectively. (nd) mutations have not been validated. Mutations leading to complete loss of Hexim1 binding (−) without affecting Cdk9 binding (+++) are shown in bold. Colours indicate the error-prone PCR reactions providing each mutation.