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Two-hybrid assay of nematode Hexim interactions with Cyclin T. (A) Yeast cells transformed with plasmids expressing wild-type or mutant C.elegans Hexim (ceHexim) and Cyclin T (cit-1.2) fused to the Gal4 DNA-binding and Gal4 activation domains, respectively, grew in LT medium. Ura3 expression induced by interaction between partners is required for growth in LTU medium (lacking uracil). (B) Like in A but using human Hexim1 and Cyclin T1 fusion proteins.
