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. 2014 Jul 5;11:57. doi: 10.1186/1742-4690-11-57

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Copyright © 2014 Jacobs et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Phosflow detection of STAT-3 and STAT-5. STAT-3 and -5 were measured by flow cytometry in CD4⁺ T cell clones co-incubated with peptide (dotted = Ant, black = Ag, grey = Ag + Ant) or mock (dashed = no peptide) pulsed B cells. Panels A and B are one representative experiment of five. Bottom panels (C) show fold change in mean fluorescence intensity (MFI) of STAT-3 and -5 from five experiments; circle = Ag, square = Ag + Ant, triangle = Ant, error bars show standard error of the mean (SEM).