Figure 3.

ATRA-induced p62/SQSTM1 upregulation is defective in NB4 cells that overexpress the IκBα (A32/36) mutant, a repressor of NF-κB activity. NB4 cells were transduced with the control lentivirus vector (NB4 control cells) or the lentivirus vector encoding for the IκBα (A32/36) mutant (NB4 MAD cells) and then were incubated for 3 days with 1 μM ATRA. (a) The levels of p62/SQSTM1 protein in control and NB4 MAD cells were assessed following immunoblot analysis using antibodies that directed against either p62/SQSTM1 or LC3-II. Levels of the wild-type (wt) IκBα mutant IκBα (A32/36) and phospho-IκBα proteins were also determined. GAPDH was used as a control. (b) Quantification of the expression of p62/SQSTM1 mRNA levels by RT-qPCR in NB4 control and NB4 MAD cells treated with 1 μM ATRA. ***P<0.001