Figure 2.

Validation of SRF expression and its subcellular localization in GC cells and tissues. (a) Representative immunostained specimens showing SRF expression in normal gastric tissues (N; n=25), primary GC tissues (C; n=28), and metastatic GC tissues (M; n=48). (b) Kaplan–Meier analysis of overall survival according to high or low SRF expression. (c) Western blot analysis of nuclear and cytoplasmic SRF levels in GC9811 and GC9811-P cells, and (d) in six groups of GC samples. Histone and tubulin were used as loading controls for nuclear and cytoplasmic extracts, respectively. (e) Immunofluorescence staining to assess subcellular SRF localization in GC9811 and GC9811-P cells, and (f) tumor samples from recipient mice