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Brd4 knockdown reduces NANOG levels in preimplantation embryos. Two-cell embryos were microinjected with control siRNA or Brd4 siRNA 1 and cultured to the late morula (a) or blastocyst (b) stage. Immunofluorescence was performed for BRD4 (red) and NANOG (green). The experiment was performed three times using at least 20 embryos per group. Representative images are shown
