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. 2014 Sep 26;15(10):17204–17220. doi: 10.3390/ijms151017204

Figure 2.

Figure 2

Sildenafil down regulates the expression of inflammatory cytokine IL-1β in the pelvic ganglia after BCNR. (A) Protein extracts were subjected to Western blot analysis for IL-1β and normalized by GAPDH with the correspondent densitometric analysis. * p < 0.05; ** p < 0.01 respect to sham. ## p < 0.01 respect to BCNR; (B) Frozen pelvic ganglia sections were immunostained with a polyclonal antibody against IL-1β followed by a biotinylated secondary antibody and streptavidin conjugated with Texas red. Red: IL-1β, Yellow: FG. White: DAPI. Magnification 100×; Bar=100 µm.