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. 2014 Sep 26;15(10):17204–17220. doi: 10.3390/ijms151017204

Figure 4.

Figure 4

Inducible nitric oxide synthase (iNOS) expression is down regulated by Sildenafiltreatment after BCNR. (A) Total protein extracts of Sham, BCNR and BCNR+SIL were subjected to Western blot studies for iNOS and normalized by GAPDH with the correspondent densitometric analysis, * p < 0.05, ** p < 0.01, respect to sham. # p < 0.05 respect to BCNR; (B) Frozen section of the pelvic ganglia were immunostained with a polyclonal antibody against iNOS followed by a biotinylated secondary antibody and Vectastain® ABC kit. 3,3'-Diaminobenzidine (DAB) was used as a chromogen and sections were counterstained with Hematoxylin. Magnification 400×. Bar= 50 μm.