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. 2014 Sep 26;15(10):17204–17220. doi: 10.3390/ijms151017204

Figure 5.

Figure 5

(A) Heme oxygyenase 1 is up-regulated by BCNR and down-regulated by Sildenafil treatment. Total RNA was extracted from 7 days Sham, BCNR and BCNR+ SIL and was subjected to real time PCR by TaqMan for HO-1 and normalized by the Ribosomal protein, large 1/3 (RPLP1/3) housekeeping gene; (B) Pelvic ganglia protein extracts were subjected to Western blot analysis for HO-1 by employing a monoclonal antibody normalized by GAPDH with the correspondent densitometric analysis; (C) Frozen pelvic ganglia sections from 7 days Sham, BCNR and BCNR+SIL were immunostained for HO-1 by employing a polyclonal antibody, with subsequent detection by DAB. No Counterstaining was performed. Representative pictures. Magnification 200×; Bar=50 µm. Image Analysis determined by integrated optical density (IOD) per cells in the pelvic ganglia *** p < 0.001; ** p < 0.01; * p < 0.05 with respect to Sham. # p < 0.05 , ### p < 0.001 with respect to BCNR.