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. 2014 Sep 26;15(10):17304–17317. doi: 10.3390/ijms151017304

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© 2014 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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(A) The expression of mRNA for Mel1aR in DP-805 cells (a human dental papilla cell line). Mel1aR mRNA expression was detected at 3 days of culture before reaching confluence (−3d), gradually increased until 3 days after reaching confluence (+3d), and gradually decreased thereafter until 3 weeks of culture (+3W); and (B) Expression of Mel1aR protein in DP-805 cells. DP-805 cells were cultured until reaching confluence. Thereafter, the expression of Mel1aR protein was analyzed by Western blot. A major band for Mel1aR was observed at ~37 kDa. The amount of protein loaded for DP-805 cells and rat brain was 80 and 100 µg, respectively.