Figure 4.

Effect of melatonin on the proliferation of DP-805 cells. DP-805 cells were seeded into 24-well culture plates at a cell density of 1 × 10⁴ cell/well and cultured in α-modified Eagle’s Medium (α-MEM) containing 1% fetal bovine serum (FBS) and a variety of concentrations (0, 1, 10, 50, 100 and 200 µM) of melatonin. The proliferation of DP-805 cells was analyzed using a crystal violet staining method. Melatonin exerted no influence on the proliferation of DP-805 cells at any concentrations not less than 1 µM and not more than 200 µM. Values are the mean ± SD.