Figure 5.
Effect of melatonin on the differentiation of DP-805 cells. DP-805 cells were plated at a cell density of 1 × 105 cells/well and cultured until they reached confluence. Thereafter, the cells were treated with various concentrations (0, 1, 10, 50, 100 and 200 µM) of melatonin for 14 days. Melatonin promoted the expression of mRNA for osteopontin (OPN) (A); bone sialoprotein (BSP) (C); dentin matrix protein-1 (DMP-1) (D) and dentin sialophosphoprotin (DSPP) (E) at concentrations equal to and more than 100 µM; The expression of mRNA for osteocalcin (OCN) (B) was also enhanced at concentrations of at least 50 µM. Values are expressed as the mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001.