Table 1.
Effect of 5-aza by high-performance liquid chromatography (HPLC) quantitation on deoxycytidine monophosphate (dCMP) and methylated dCMP (mdCMP) in immortalized ovarian surface epithelium (IOSE), A2780 and CP70 cell lines.
| Sample | Treatment | Retention Time (min) | Ratio of Methylation a | |
|---|---|---|---|---|
| dCMP | mdCMP | |||
| Standard | 3.6 ± 0.1 | 3.9 ± 0.1 | ||
| IOSE | None | 3.6 ± 0.1 | 4.1 ± 0.1 | 4.3% ± 1.7 |
| 5-Aza | 3.6 ± 0.1 | 3.9 ± 0.1 | 4.2% ± 0.8 | |
| A2780 | None | 3.5 ± 0.1 | 4.0 ± 0.1 | 5.4% ± 2.4 |
| 5-Aza | 3.6 ± 0.1 | 4.1 ± 0.1 | 1.3% ± 0.1 | |
| CP70 | None | 3.6 ± 0.1 | 4.0 ± 0.1 | 7.7% ± 2.0 |
| 5-Aza | 3.6 ± 0.1 | 4.1 ± 0.1 | 4.8% ± 0.6 | |
a Ratio of methylation was determined as follows. AmdCMP/(AdCMP + AmdCMP), A = integration of the absorption at 254 nm. The reported values were calculated from three independent measurements.