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17β-Estradiol (E2)- and 17α-ethinylestradiol (EE2)-induced Ru-Vg transcription. Quantitative reverse transcription (RT)-PCR was performed on equal amounts of total RNA isolated from Korean rose bitterling hepatopancreas. Transcript levels were quantified relative to β-actin. Expression levels were calculated relative to the level of Ru-Vg in an unstimulated sample, which was arbitrarily defined as 1. PCR reactions were performed at least in triplicate.
