Figure 6.

Functional analysis of the 5'-flanking regions of the Ru-Vg gene. Cells were seeded in 24-well culture plates and transfected with a Ru-Vg luciferase reporter vector and a β-galactosidase expression plasmid, together with an ERα expression plasmid. At 24 h after transfection, the cells were incubated in the presence or absence of E2 or EE2 for 16 h. At 48 h post-transfection, the cells were lysed and subjected to a luciferase reporter assay. Data are representative of two independent experiments and are presented as the mean ± SD (n = 3). * indicates p value < 0.05 with respect to the mock transfectants was deemed to indicate statistical significance.