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. 2014 Oct 20;15(10):18840–18855. doi: 10.3390/ijms151018840

Figure 5.

Figure 5

Effects of fucoidan on the activation of MAPKs and the expression of osteoclast-specific transcription factors by RANKL. BMMs were serum-starved for 5 h, pre-treated with fucoidan (5 μg/mL) or vehicle (PBS) for 4 h and then stimulated with RANKL (100 ng/mL). Cell lysates were collected, and equal amounts of protein were separated by 10% SDS-PAGE and analyzed by western blotting using (A) c-Fos and NFATc1 antibodies; and (B) anti-phospho-ERK, anti-phospho-p38, and anti-phospho JNK antibodies.