Table 2.
Immunofluorescent staining in a spinal cord injury rat model.
| Antibody | Antigen | Host | Company | Catalog# | Dilution |
|---|---|---|---|---|---|
| Primary Antibody | |||||
| NeuN | Neuron | Mouse | Millipore | MAB377 | 1:400 |
| GFAP | Astrocyte | Mouse | Abcam | ab4648 | 1:200 |
| TUNEL | Apoptosis | Clontech | 630108 | 1:200 | |
| HSP 72 | HSP 72 | Rabbit | Abgent | AJ1380a | 1:200 |
| DAPI | Nucleic acids | Invitrogen | D1306 | 1:20,000 | |
| Secondary Antibody (conjugation) | |||||
| Mouse IgG (Alexa Fluor 568) | Mouse IgG | Goat | Invitrogen | A11031 | 1:200 |
| Rabbit IgG (Alexa Fluor 488) | Rabbit IgG | Goat | Invitrogen | A11034 | 1:200 |