Table 1.
Parameters for the mitotic spindle assembly model.
| Parameter | Human | Budding Yeast | Remarks | |
|---|---|---|---|---|
| Rate constants: | k1 | 1.00 × 10−3 µM−1s−1 | 4.83 × 10−5 µM−1s−1 | [34]/[32] |
| k2 | 2.00 × 10−1 µM−1s−1 | 3.00 × 10−1 µM−1s−1 | [35]/[32] | |
| k3 | 1.00 × 102 µM−1s−1 | 3.00 × 10−3 µM−1s−1 | [28]/[32] | |
| k4 | 1.00 × 10−2 µM−1s−1 | NA | [28] | |
| k5 | 1.00 × 102 µM−1 s−1 | NA | [28] | |
| k−1 | 1.00 × 10−2 s−1 | 4.83 × 10−6 s−1 | [28]/[32] | |
| k−2 | 2.00 × 10−1s−1 | 4.50 × 10−1 s−1 | [35]/[32] | |
| k−3 | 0.00 s−1 | 2.00 × 10−1 µM−1s−1 | [28]/[32] | |
| k−4 | 3.00 × 10−2 s−1 | NA | [28] | |
| k−5 | 0.00 s−1 | NA | [28] | |
| Initial amount: | ||||
| Cdc20 | 0.22 µM | 0.1 µM | [36,37]/[32] | |
| O-Mad2 | 0.15 µM | 0.2 µM | [35]/[32] | |
| Cdc20:C-Mad2 | 0 µM | 0 µM | [29]/[33] | |
| Mad1:C-Mad2 | 0.05 µM | 0.00616 µM | [31]/[32] | |
| Mad1:C-Mad2:Mad2* | 0 µM | 0 µM | [31]/[32] | |
| Cdc20:C-Mad2:Mad2* | 0 µM | NA | [31] | |
| Diffusion constants: | ||||
| Cdc20 | 19.5 µm2s−1 | 19.5 µm2s−1 | [38] | |
| O-Mad2 | 0.0 − 50.0 µm2s−1 | 0.0 − 50.0 µm2s−1 | ||
| Cdc20:C-Mad2 | 0.0 − 14.0 µm2s−1 | 0.0 − 14.0 µm2s−1 | ||
| Mad1:C-Mad2 | 0 | 0 | ||
| Mad1:C-Mad2:Mad2* | 0 | 0 | ||
| Cdc20:C-Mad2:Mad2* | 0.0 − 11.0 µm2s−1 | NA | ||
| Environment: | ||||
| radius of the kinetochore | 0.1 µm | 0.015 µm | [39]/[40,41] | |
| radius of the cell | 10 µm | 2 µm | [42]/[43] |