Table 1.
Primers and probe sequences used for quantitative Real-Time PCR analysis in A. annua cell cultures.
| Gene | Sequence | Accession Number | |
|---|---|---|---|
| HMGR | Forward Reverse Probe | 5'-CATGCTTGAACCTACTTGGAGTCA-3' 3'-CAACACCGAACCAGCAACTATC-5' 5'-TGCGTGCATAGAATCACCAGGCTCA-3' | AF142473.1 |
| DXR | Forward Reverse Probe | 5'-CCCGTCTTGATCTTTGCAAGTT-3' 3'-GCAGAACAGCCAAATGCATT-5' 5'-AAGCACCGGACAACGTGAAATACCCG-3' | AF182287.2 |
| IDI * | Forward Reverse Probe | 5'-CAGACTTAGGTGAGGAGGGTCTCA-3' 3'-CCCACCACTTGAACAAGAAATTG-5' 5'-CTGTCGCCGTGGTTCAGGATTGTTG-3' | DQ666334.1 |
| FDS | Forward Reverse Probe | 5'-TCACCGCCGAATTGTTCA-3' 3'-TGCTTGTCAAGATCCTCTCCAA-5' 5'-ACTCATTTTACCTTCCAGTTGCCTGTGCAC-3' | U36376.1 AF112881.1 |
| GGDS | Forward Reverse Probe | 5'-AGGTTGTTTGCTGAGGAGTTGTT-3' 3'-CCACACCCCTCTCTGATTCAA-5' 5'-CCGAGGCGAAGCAGCAGTTGG-3' | EY076993.1 EY082045.1 |
| PS * | Forward Reverse Probe | 5'-GGGCGTATGTAAGCAAACCAA-3' 3'-CTTGATGATGCTGGTACAAGTGATT-5' 5'-AAGATAGTTGCTTTGCCTCTGGCATATGCA-3' | EY033375.1 |
| LCYB * | Forward Reverse Probe | 5'-TTTAGAAGGCACAAGACGGTTTT-3' 3'-AAAGTGAATAACTCAGGCAGAAACAA-5' 5'-ACCTCGTTACTGGCATGGGTTCTTGTCATC-3' | EY092112.1 |
| β-action | Forward Reverse Probe | 5'-CCATTGGTGCTGAGAGGTTCA-3' 3'-GCAGCTTCCATTCCGATCA-5' 5'-TGCCCTGAGGTCTTGTTCCAACCTTC-3' | EU531837.1 |
* A. annua IDI, (DQ666334.1) PS (EY033375.1) and LYC-B (EY092112.1) sequences were identified by searching in the A. annua EST sequences database on the basis of Helianthus annuus homologous genes.