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. 2014 Oct 23;15(10):19253–19264. doi: 10.3390/ijms151019253

Figure 3.

Figure 3

DRAM1 knockdown inhibits autophagy activation and aggravates OGD/R-induced Neuro-2a cell injury. Neuro-2a cells were transfected with negative siRNA or DRAM1 siRNA and then subjected to 4 h OGD and 12 h reperfusion. (A) Neuro-2a cells were transfected with negative siRNA or DRAM1 siRNA; (B) The quantitative results from (A); (C) Cell viability was assessed using the LIVE/DEAD viability/cytotoxicity kit; (D) Relative protein expression levels. At the indicated pretreatment, Neuro-2a cells were collected, and protein was extracted to assess LC3, p62, and LAMP2 expression through Western blot analysis. β-Actin was used as an internal control; and (E) The quantitative results from (D). β-Actin was used as an internal control (* p < 0.05 vs. control, # p < 0.05 vs. ODG/R 12 h group, n = 6).