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. 2014 Jul 7;14:226. doi: 10.1186/1472-6882-14-226

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Copyright © 2014 Saleh et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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AVO-b modulates the expression of Bcl-2 family proteins, and promotes translocation of Bax and t-Bid to the mitochondria of HL-60 cells in a dose-dependent manner. Whole cell extracts (50 μg), mitochondrial (30 μg) and cytosolic (50 μg) fractions were prepared from HL-60 cells treated with AVO-b as described above. W.E were probed with antibodies against Bak, Bcl-2 and Bcl-xL proteins. Mit. fractions were immuneblotted with antibodies against t-Bid, Bax and VDAC, while Cyt. fractions were probed with antibodies for Bid and Bax. The same Western blots for the Cyt. fractions and W.E were probed with antibodies to α-tubulin and β-actin, respectively.