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. Author manuscript; available in PMC: 2014 Nov 11.
Published in final edited form as: Mol Microbiol. 2010 Feb 10;76(1):104–119. doi: 10.1111/j.1365-2958.2010.07083.x

Fig. 2. The agu2ABCA′ and aguBA operons of PA14 and select mutants.

Fig. 2

A. The agu2ABCA′ operon contains two agmatine deiminase genes agu2A and agu2A′. The black box in gene agu2A′ designates a tat secretion peptide. The promoter region for this operon is denoted with arrows between agu2R and agu2A and is shown in B. The aguBA operon has been described (Nakada et al., 2001) and is shown for comparison. Open reading frames shown in approximate scale to relative base pair length. The agu2ABCA′::Tet construct (not shown) is the same as the agu2ABCA′::Gm except a TetR cassette replaces the GmR cassette.

B. The intergenic region between agu2R and agu2A contains two putative promoters designated by thin arrows. The transcription starts sites (TSS) are designated as +1 and the TSS for agu2A has been experimentally verified.