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. Author manuscript; available in PMC: 2014 Nov 11.
Published in final edited form as: Mol Microbiol. 2010 Feb 10;76(1):104–119. doi: 10.1111/j.1365-2958.2010.07083.x

Fig. 3. Agu2A and Agu2A′ are agmatine deiminase enzymes with a conserved C-terminal cysteine active site.

Fig. 3

A. Coomassie stained gel with crude cell lysates (1–5) or nickel column purified fractions (6–8) of arabinose induced TOP10 cultures harbouring plasmids expressing the following proteins: 1. Agu2A, 2. Agu2A′, 3. Agu2A′:C364G, 4. Agu2A′ with no arabinose induction, 5. TOP10 cloning strain, 6. Agu2A eluate, 7. Agu2A′ eluate, 8. Agu2A′ C364G eluate. See Table 1 for corresponding enzymatic activities.

B. Comparison of the C-terminal amino acid sequences of members of the guanidino modifying enzymes found in P. aeruginosa. Conserved amino acids are highlighted and have been described (Shirai et al., 2006).