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. Author manuscript; available in PMC: 2014 Nov 11.
Published in final edited form as: Mol Microbiol. 2010 Feb 10;76(1):104–119. doi: 10.1111/j.1365-2958.2010.07083.x

Fig. 4. Agu2A′ is secreted into the periplasm through the twin-arginine translocase system.

Fig. 4

A. N-terminal amino acid sequence of Agu2A′ and its secretion mutant demonstrating the predicted twin arginine consensus sequence (critical amino acids are highlighted) and the proposed cleavage site in the wild type strain (arrow).

B. Western blot to C-terminal 6-His tag of protein fractions from Agu2A′ or Agu2A′ R3S, R4G expression vectors induced with arabinose in strain PAO1. Cell fractionation is designated as 1. Inner membrane, cytosol, 2. Outer membrane, periplasm, 3. Supernatant. Each lane was loaded with 15 μg of protein from the various fractions. See Results section for description of the enzymatic assays used to ensure proper fractionation.