Figure 7.

Proteomic and redox analysis of aconitase from gastrocnemius muscle of adult and old mice. (A) PEAKS label-free proteomic quantification of aconitase from a shotgun proteomics of gastrocnemius muscle from adult and old mice. (B) Western blotting validation of aconitase expression. (C) Aconitase enzymatic activity showing a decrease in muscle from old mice. (D) Redox quantification using Skyline of reversible oxidation state of individual Cys residues. (E) Structure of aconitase with Cys residues detected and quantified (red) and coordinating iron (green). (F) Schematic of aconitase oxidation.