Table II.

Oligonucleotide primer sequences used in this study.

Primer	Sequence (5′-3′)	Restriction enzyme
Sly_upF	GGGGGAAGCTTCTAGTCGGGGGAGTTTTTGTG	HindIII
Sly_upR	GGGGGGGTCGACTTAATATCTTGTTTGGAATCTG	SalI
Cm_F	GCGGTCGACTAATTCGATGGGTTCCGAGG	SalI
Cm_R	CGCGGATCCCACCGAACTAGAGCTTGATG	BamHI
Sly_downF	GGGGGGATCCCATGGGAGTGGTGGAGAACAGT	BamHI
Sly_downR	GGGGGGGGAATTCTTGGCCCGAATACCGACAG	EcoRI

Sly, suilysin; up, upstream; down, downstream; F, forward; R, reverse; Cm, chloramphenicol; underlined, restriction sites.