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. 2014 Sep 18;10(6):2912–2916. doi: 10.3892/mmr.2014.2582

Figure 1.

Figure 1

Nucleotide variation altered the predicted secondary structure of pre-miR-34a and reduced mature miR-34a expression. (A) Predicted structure of 110 nt pre-miR-34a. Single nucleotide replacement by A caused changes in the size of one loop, one stem structure and an increase in entropy. (B) miR-34a-5p expression levels generated by different genotypes of pre-miR-34a were detected using quantitative polymerase chain reaction. miR levels were normalized against U6 small nuclear RNA. The Y-axis displays the relative expression of miR-34a normalized to U6. Results are expressed as the mean±standard error of the mean. *P<0.05, **P<0.01. miR, micro RNA.