Figure 1.

Schematic diagram of the constructs used for the suppression of β-conglycinin soybean seeds (A) and expression of 11 kDa δ-zein (B). The RNAi cassette contains a 314 bp region of the α′ subunit of the β-conglycinin cloned in an inverted repeat orientation and separated by the intron from pKannibal. The RNAi construct is under the control of the soybean β-conglycinin α′-promoter while the expression of 11 kDa δ-zein is under the control of soybean glycinin promoter (Kim and Krishnan, 2004). The constructs also contain a gene expression cassette that includes the cauliflower mosaic virus 35S promoter, the bar-coding region and the 3′ region of the nopaline synthase gene (nos).