Figure 2.

Suppression of β-conglycinin accumulation in transgenic soybean lines. Total seed proteins from wild-type (lane 1) and from seven independent RNAi transgenic lines (lanes 2–8) were fractionated in duplicate gels by 10% SDS-PAGE and stained with Coomassie Blue (A) or transferred to nitrocellulose membranes. The membrane was probed with the β-conglycinin antibodies (B). Immunoreactive proteins were identified using anti rabbit IgG-horseradish peroxidase conjugate followed by chemiluminescent detection. Note the absence of α′, α, and β-subunits of β-conglycinin in some of the RNAi lines.