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. 2014 Nov 11;5:633. doi: 10.3389/fpls.2014.00633

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Copyright © 2014 Kim, Jez and Krishnan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of sulfur nutrition on the accumulation of the 11 kDa δ-zein in transgenic soybean seeds. Total seed proteins from 11 kDa δ-zein expressing line and the 11 kDa δ-zein introgressed into the β-conglycinin knockdown soybean line (SAM) that were grown hydroponically in presence of 0.5 or 2 mM magnesium sulfate were fractionated in duplicate gels by 15% SDS-PAGE and stained with Coomassie Blue (A) or transferred to nitrocellulose membranes. The membrane was probed with the 11 kDa δ-zein specific antibodies (B). Immunoreactive proteins were identified using anti rabbit IgG-horseradish peroxidase conjugate followed by chemiluminescent detection.