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. Author manuscript; available in PMC: 2014 Nov 11.
Published in final edited form as: Cancer Res. 2012 Aug 23;72(20):5317–5327. doi: 10.1158/0008-5472.CAN-12-1972

Figure 4. Survival of Nf1 mutant SCPs is dependent on TGFβ and AKT.

Figure 4

SCP spheres treated with (A) anti-TGFβ antibody (B) TGFβR1 inhibitor, SB 431542 and (C) AKT inhibitor, MK-2206 or (D) shAKT compared to NT. Spheres counted after treatment. (E) E12.5 DRGs stained with anti-phospho-AKT. Adjacent sections stained with anti-neurofilament (to highlight neurons in DRG). Scale bar: 50µm. (F) Quantification of staining intensity for phospho-AKT in DRG sections. (G) qRT-PCR, TGFβ1 mRNA expression in Nf1−/− SCP spheres after treatment. (H) Model of TC21 dependent TGFβ autocrine loop in Nf1−/− SCPs.