Figure 3.

The expression of macrophage markers is exacerbated in injured nerves in the absence of ApoD. GFAP protein expression normalized to β-actin (A) and Emr1/F4-80 mRNA expression (B) in intact nerves and in the distal region of injured nerves of WT and ApoD-KO mice at 7d-PCI. (C) Representative examples of CR3/Cd11b immunohistochemistry in sagittal sections of the injury region of crushed sciatic nerves. Arrows point to CR3-positive macrophages. Calibration bars: 50 μm. (D) Immunoblot analysis of the CR3/Cd11b protein in the region distal to the lesion of WT and ApoD-KO mice at 7d-PCI. Statistical differences were assayed by Student's t-Test (A,D) and Mann-Whitney U-test (B). Asterisks show statistically significant differences (P < 0.05).