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. 2014 Jun 5;13(11):4526–4534. doi: 10.1021/pr5003593

Figure 3.

Figure 3

Analysis of the N40K C-terminus by LC–SRM. (A) Work flow of the method. (B) Identification and size confirmation of purified recombinant U1-70K by Western blotting, followed by band excision and in-gel digestion. (C) Identification of a N40K–containing gel band by Western blotting. The 10 kDa ladder was also used for precise alignment of immunoblotting images with stained gel. (D) The intensity ratios of light/heavy (L/H) of U1-70K peptide pairs, leading to the predicted cleavage region.