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Determination of N40K C-terminus by Western blotting. (A) Expression of U1-70K N-terminal truncations in HEK293 cells. (B) Analysis of N40K in AD and three recombinant truncations. (C) Diagram of U1-70K domains and predicted N40K cleavage region. (D) N40K truncations covering all possible cleavage products with different C-terminal residues.
