Table 1. Relative Protein Abundances in Control and FECD Tissuea.
| control tissue | % | FECD tissue | % | ||
|---|---|---|---|---|---|
| 1 | Transforming growth factor-beta-induced protein ig-h3 | 38.6 ± 10.0 | 1 | Transforming growth factor-beta-induced protein ig-h3 | 49.5 ± 8.8 |
| 2 | Collagen alpha-3(IV) chain | 9.9 ± 1.8 | 2 | Collagen alpha-2(VIII) chain | 10.7 ± 5.0 |
| 3 | Collagen alpha-2(VIII) chain | 9.1 ± 3.6 | 3 | Collagen alpha-3(IV) chain | 9.3 ± 2.7 |
| 4 | Collagen alpha-2(I) chain | 8.1 ± 11.7 | 4 | Collagen alpha-1(VIII) chain | 2.7 ± 1.1 |
| 5 | Collagen alpha-1(VIII) chain | 2.6 ± 0.9 | 5 | Clusterin | 2.6 ± 0.6 |
| 6 | Collagen alpha-4(IV) chain | 2.5 ± 2.1 | 6 | Ig kappa chain C region | 1.5 ± 0.4 |
| 7 | Collagen alpha-1(II) chain | 1.9 ± 1.6 | 7 | Fibulin-5 | 1.5 ± 0.2 |
| 8 | Keratin, type II cytoskeletal 1 | 1.8 ± 0.8 | 8 | Prolactin-inducible protein | 1.4 ± 1.7 |
| 9 | Clusterin | 1.7 ± 0.6 | 9 | Apolipoprotein D | 1.3 ± 0.2 |
| 10 | Hemoglobin subunit beta | 1.7 ± 1.5 | 10 | Serine protease HTRA1 | 1.3 ± 0.6 |
| 11 | Fibrillin-1 | 1.6 ± 0.9 | 11 | Collagen alpha-2(I) chain | 1.3 ± 0.7 |
| 12 | Collagen alpha-5(IV) chain | 1.5 ± 0.6 | 12 | Collagen alpha-5(IV) chain | 1.2 ± 0.2 |
| 13 | Metalloproteinase inhibitor 3 | 1.5 ± 2.1 | 13 | Sushi repeat-containing protein SRPX2 | 1.2 ± 0.8 |
| 14 | Fibulin-5 | 1.3 ± 0.7 | 14 | Metalloproteinase inhibitor 3 | 1.2 ± 1.0 |
| 15 | Keratin, type I cytoskeletal 9 | 1.3 ± 0.8 | 15 | Collagen alpha-4(IV) chain | 1.1 ± 0.3 |
| 16 | Sushi repeat-containing protein SRPX2 | 1.2 ± 1.0 | 16 | Keratocan | 1.0 ± 0.6 |
| 17 | Thrombospondin-1 | 1.2 ± 0.1 | 17 | C-type lectin domain family 11 member A | 0.9 ± 0.1 |
| 18 | Serine protease HTRA1 | 1.1 ± 0.5 | 18 | Laminin subunit beta-1 | 0.9 ± 0.1 |
| 19 | Apolipoprotein E | 1.0 ± 1.0 | 19 | Thrombospondin-1 | 0.8 ± 0.4 |
| 20 | Laminin subunit beta-1 | 1.0 ± 0.6 | 20 | Keratin, type II cytoskeletal 2 epidermal | 0.8 ± 0.2 |
| 21 | Prolactin-inducible protein | 1.0 ± 1.2 | 21 | Collagen alpha-1(XII) chain | 0.7 ± 0.3 |
| 22 | Apolipoprotein D | 1.0 ± 0.2 | 22 | Collagen alpha-2(IV) chain | 0.7 ± 0.4 |
| 23 | Biglycan | 0.9 ± 1.0 | 23 | Keratin, type II cytoskeletal 1 | 0.6 ± 0.3 |
| 24 | Collagen alpha-1(XII) chain | 0.9 ± 0.5 | 24 | Apolipoprotein E | 0.6 ± 0.3 |
| 25 | Keratin, type II cytoskeletal 2 epidermal | 0.9 ± 0.5 | 25 | Basement membrane-specific heparan sulfate proteoglycan core protein | 0.6 ± 0.2 |
| 26 | C-type lectin domain family 11 member A | 0.8 ± 0.3 | 26 | Collagen alpha-6(IV) chain | 0.6 ± 0.2 |
| 27 | Collagen alpha-2(V) chain | 0.8 ± 0.8 | 27 | Thrombospondin-4 | 0.6 ± 0.2 |
| 28 | Thrombospondin-4 | 0.8 ± 0.5 | 28 | Fibrillin-1 | 0.6 ± 0.2 |
| 29 | Collagen alpha-1(IV) chain | 0.7 ± 0.1 | 29 | Serum albumin | 0.6 ± 0.2 |
| 30 | Collagen alpha-2(IV) chain | 0.7 ± 0.2 | 30 | Prostaglandin-H2 d-isomerase | 0.6 ± 0.1 |
| 31 | Keratin, type I cytoskeletal 10 | 0.7 ± 0.5 | 31 | Collagen alpha-1(IV) chain | 0.6 ± 0.3 |
| 32 | Myocilin | 0.7 ± 0.8 | 32 | Serum amyloid P-component | 0.6 ± 0.2 |
| 33 | Collagen alpha-6(IV) chain | 0.7 ± 0.3 | 33 | Keratin, type I cytoskeletal 9 | 0.5 ± 0.2 |
| 34 | Collagen alpha-1(III) chain | 0.7 ± 0.2 | 34 | Collagen alpha-1(II) chain | 0.4 ± 0.2 |
| 35 | Basement membrane-specific heparan sulfate proteoglycan core protein | 0.6 ± 0.2 | 35 | Myocilin | 0.4 ± 0.2 |
| 36 | Prostaglandin-H2 d-isomerase | 0.6 ± 0.4 | 36 | EGF-containing fibulin-like extracellular matrix protein 1 | 0.3 ± 0.2 |
| 37 | Serum amyloid P-component | 0.5 ± 0.2 | 37 | Histone H4 | 0.3 ± 0.1 |
| 38 | Keratocan | 0.4 ± 0.2 | 38 | Fibronectin | 0.3 ± 0.1 |
| 39 | Periostin | 0.3 ± 0.3 | 39 | Laminin subunit alpha-5 | 0.3 ± 0.04 |
| 40 | Laminin subunit alpha-5 | 0.3 ± 0.1 | 40 | Keratin, type I cytoskeletal 10 | 0.3 ± 0.1 |
| 41 | Lysyl oxidase homologue 4 | 0.3 ± 0.1 | 41 | Collagen alpha-2(V) chain | 0.3 ± 0.1 |
| 42 | EGF-containing fibulin-like extracellular matrix protein 1 | 0.3 ± 0.1 | 42 | Agrin | 0.2 ± 0.1 |
| 43 | Collagen alpha-3(VI) chain | 0.3 ± 0.1 | 43 | Alpha-1-antitrypsin | 0.2 ± 0.1 |
| 44 | EMILIN-1 | 0.3 ± 0.1 | 44 | Collagen alpha-3(VI) chain | 0.2 ± 0.1 |
| 45 | Nidogen-1 | 0.2 ± 0.03 | 45 | EMILIN-1 | 0.2 ± 0.1 |
| 46 | Laminin subunit gamma-1 | 0.2 ± 0.02 | 46 | Laminin subunit gamma-1 | 0.2 ± 0.1 |
| 47 | Alpha-1-antitrypsin | 0.2 ± 0.03 | 47 | Nidogen-1 | 0.2 ± 0.1 |
| 48 | Agrin | 0.2 ± 0.03 | 48 | Keratin, type I cytoskeletal 12 | 0.2 ± 0.1 |
| 49 | Collagen alpha-1(III) chain | 0.2 ± 0.2 | |||
| 50 | Cadherin-1 | 0.2 ± 0.1 | |||
| 51 | Olfactomedin-like protein 1 | 0.1 ± 0.04 |
a
The relative abundance of the proteins was calculated as the average MS intensity (area) for the three most intense peptides from each protein divided by the total sum of the average signal (area) for all quantified proteins. Average ratios and standard deviations were calculated based on the biological replicates, and only proteins quantified in a minimum of half of the biological replicates are reported.