Figure 1.

Experimental design for iTRAQ-based quantitative proteomic analysis. The whole experiment was repeated (two batches) and each SCX fraction was analyzed twice using nanoRP-LC–MS/MS (Replicates 1 and 2). Thus, there are two biological replicates and two technical replicates. The hard lenses were placed over the right eye of mice from two groups (myopic group and atropine-treated group) on day 10 and remained in place until postnatal day 52. Atropine-treated group received one drop of 1% atropine sulfate topically. The treatment was started on postnatal day 24.