Figure 2.

Atropine sulfate (pan muscarinic antagonist) and minus lens-treated B6 mice ocular biometry measurements were plotted against induction period (weeks). This graph represents the ocular biometry of C57BL/6 (B6) mice with −15 D spectacle lens alone or −15 D spectacle lens with atropine treatment. The lens was applied at postnatal day 10 (before eye opening), and the drug treatment was started after 2 weeks of minus lens wearing. The ocular biometry measurements were measured using OLCI-AcMaster (in vivo: accuracy ±10 μm), and refraction (diopters) was measured by automated infrared photorefractor at 2, 4, and 6 weeks after induction of myopia. The refraction (A) and axial length (B) changes were significant after 4 weeks and 6 weeks of myopia induction. Spectacle lens treatment induced myopia and caused elongation of the globe. However, the axial length was significantly reduced after receiving atropine. The lens-treated eyes that received atropine were shifted from myopic to hyperopic refractions, similar to those of naïve control eyes. There was no significant difference seen in the control and fellow eyes. Data were represented as mean ± S.D.; * represents significance level p < 0.01.