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. 2014 Nov 5;9:5131–5141. doi: 10.2147/IJN.S71074

Figure 4.

Figure 4

Detection of autophagic vacuoles induced by Nano-SiO2 via MDC staining.

Notes: (A) Confocal microscopy images of MDC staining after HUVECs were exposed to 50 μg/mL of Nano-SiO2 for 24 hours. (B) Quantification of MDC staining by fluorescent intensity analysis. Student’s t-test is used for the data analysis. Data are expressed as the mean ± standard deviation from three independent experiments (*P<0.05).

Abbreviations: Nano-SiO2, silica nanoparticles; MDC, monodansylcadaverine; HUVECs, human umbilical vein endothelial cells.