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. 2014 Nov 11;12(11):e1001993. doi: 10.1371/journal.pbio.1001993

Figure 7. Incubation with the NG2 LNS domains results in an increased expression of c-fos in cortical neurons.

Figure 7

(A–C) Acute slices of the somatosensory cortex of NG2−/− mice were pre-incubated (1 h) with the NG2 LNS domains (LNS-AP) (B) or the AP control protein (A) and subsequently stimulated with glutamate for 10 min. Immunofluorescence staining was performed for the activity-dependent early gene c-fos (in red) and the neuronal marker NeuN+ (green). Incubation with the NG2 LNS domains resulted in more c-fos+ neurons compared to slices incubated with the control AP protein (C). c-fos+ cell numbers were related to NeuN+ cell numbers (c-fos+/NeuN+ cell numbers) from each double stained slice. Cell numbers of representative cortical areas as shown in (A) and (B) were counted from seven different animals (n = 7) for each incubation condition. The incubation conditions (LNS-AP and AP) were compared from the same individual mouse. c-fos/NeuN numbers were compared between the two experimental conditions (paired Student's t test).