Figure 4.

Effect of AM on nuclear intensity, MMP, permeability, and cytochrome c release.

Notes: (A) Representative images of MDA-MB-231 cells treated with medium alone or 5 μg/mL, 10 μg/mL, or 20 μg/mL of AM, and stained with Hoechst 33342 for nuclear intensity, cell permeability dye, MMP, and cytochrome c. The images from each row were obtained from the same field of each sample (magnification 20×). (B–E) Average fluorescence intensities of Hoechst 33342, cell permeability dye, MMP, and cytochrome c, respectively, in MDA-MB-231 cells treated with AM or standard drug tamoxifen. Data were mean ± standard deviation of fluorescence intensity readings measured from different photos taken (*P<0.05).

Abbreviations: AM, α-mangostin; MMP, mitochondrial membrane potential.