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. 2014 Nov 11;9(11):e111860. doi: 10.1371/journal.pone.0111860

Figure 1. MCL1 is a direct target of miR-107.

Figure 1

A, the expression level of miR-107 in HeLa and SiHa cells was significantly altered following transfection with either pri-miR-107 or ASO-miR-107 expression constructs as determined by qRT-PCR using U6 snRNA for normalization. B, the intensity of EGFP fluorescence in HeLa cells transfected with pri-miR-107 was decreased after 48 h and increased following transfection with ASO-miR-107. C, pri-miR-107 and ASO-miR-107 had no effect on the intensity of EGFP fluorescence in cells transfected with the 3′-UTR mutant vector. The mRNA (D) or protein (E) levels of MCL1 in HeLa and SiHa cells decreased or increased when compared with the control group when pri-miR-107 was overexpressed or blocked, respectively (*, p<0.05, **, p<0.005).