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. 2014 Nov 11;9(11):e111860. doi: 10.1371/journal.pone.0111860

Figure 2. miR-107 represses cell proliferation and migration and invasiveness.

Figure 2

Cervical cancer cells were transfected with either pri-miR-107 or ASO-miR-107. Cell viability was determined at 24, 48, and 72 h after seeding in 96-well plates using the MTT assay. A, the histogram shows the data at the time point of 48 h. All three data points showed a significant difference. B cervical cancer cells were transfected with pri-miR-107 or ASO-miR-107 and then seeded in 12-well plates. For the colony formation assay, the cells were stained with 2% crystal violet solution, and a representative image is shown. C and D, migration and invasion assays were performed with HeLa and SiHa cells transfected with either pri-miR-107 or ASO-miR-107. Representative images and randomly selected fields are shown. Knockdown of MCL1 suppresses proliferation, migration, and invasiveness of cervical cancer cells. E, MCL1 protein level was measured by Western blotting 48 h after transfection of siR-MCL1 into HeLa and SiHa cells. GAPDH was used as loading/transfer control (Ctrl) and for normalization of values. F and G, the effects of MCL1 knockdown on cell viability (F) were determined using the MTT assay, and cell proliferation was determined using the colony formation assay (G). H and I, changes in cell migration and invasiveness induced by siR-MCL1 were determined by migration and invasion assays. (*, p<0.05; **, p<0.005).