Table 1. List of proteins screened for effects on chromatin compaction.
NET | alt name | tag | fusion to | phenotype |
4 | Tmem53 | HA | N-terminus | no effect |
5* | Tmem201/Samp1 | mRFP | C-terminus | no effect |
8 | LPGAT1 | HA | N-terminus | no effect |
13 (n) | SMPD4 | mRFP | C-terminus | no effect on condensation, but many apoptotic cells |
14 (n) | WDR33 | HA | N-terminus | no effect |
20 | FAM105A | mRFP | C-terminus | no effect |
23 | Tmem173 | mRFP | C-terminus | strong condensation |
23 | Tmem173 | HA | strong condensation | |
24 | ERIGIC1 | mRFP | C-terminus | no effect |
25 | LEMD2 | HA | N-terminus | no effect |
26 | Tmem14c | HA | N-terminus | no effect |
29 | Tmem120 | mRFP | C-terminus | no effect |
30 | MOSPD3 | mRFP | C-terminus | some condensation, but cells very sick |
33 | SCARA5 | mRFP | C-terminus | no effect |
34 | SLC39A14 | mRFP | C-terminus | some condensation and apoptosis |
35 (n) | KIAA1967 | HA | N-terminus | no effect |
37 | KIAA1161 | mRFP | C-terminus | no effect |
38 | ALG2 | mRFP | C-terminus | no effect |
39 | PPAPDC3 | HA | N-terminus | no effect |
43 (n) | NAT10 | mRFP | C-terminus | no effect |
45 | DAK | mRFP | C-terminus | no effect |
46 | SLC22A24 | mRFP | C-terminus | no effect |
47 | TM7SF2 | mRFP | C-terminus | no effect |
49 (n) | NOC4L | mRFP | C-terminus | no effect |
50 | DHRS7 | mRFP | C-terminus | no effect |
51 | C14orf1 | mRFP | C-terminus | no effect |
52t | HA | N-terminus | no effect | |
54 (n) | mRFP | C-terminus | no effect | |
55 | APH1B | mRFP | C-terminus | no effect |
56 | Dullard | HA | N-terminus | no effect |
59 | NCLN | mRFP | C-terminus | no effect |
62 | MCAT | HA | N-terminus | no effect |
*shorter splice variant.
truncated version, (n) indicates NETs that targeted poorly to the NE in these cells or appeared to be nucleoplasmic.