Figure 10. UV illumination of sEGFR prevents binding of anti-EGFR neutralizer antibody LA1 to its target antigen.

In each well, exactly 1.4 µg of non illuminated (“No-UV”), UV illuminated for 75 min (“UV”) and negative control (“NC”) sEGFR samples was loaded. Samples loaded on well 1–3 are duplicates of samples 4–6, but were treated independently after UV illumination. The intensity profile along the wells shows that signal is observed in the wells with non-illuminated protein but no signal is present in the wells containing UV illuminated protein samples.