Table 1. Lifetimes (τi) and pre-exponential values (αi) for sEGFR (negative control and UV illuminated samples) at pH 7.5 obtained by a nonlinear fit (see Figure 6 for example, negative control sEGFR) using the ISS software.
Lifetime (ns) | Intensity fraction | Pre-exponential | |||
sEGFR Dark for 75 min (negative control, NC) | |||||
τ1 | 1.04±0.06 | f 1 | 0.258±0.02 | α1 | 24.8 |
τ2 | 2.74±0.1 | f 2 | 0.531±0.01 | α2 | 19.4 |
τ3 | 6.23±0.2 | f 3 | 0.211 Fixed | α3 | 3.38 |
<τ> f weighed | 3.04 | ||||
sEGFR Illum. for 75 min (at 280 nm) | |||||
τ1 | 1.02±0.04 | f 1 | 0.306±0.02 | α1 | 30 |
τ2 | 2.88±0.1 | f 2 | 0.53±0.01 | α2 | 18.4 |
τ3 | 7.0±0.2 | f 3 | 0.164 Fixed | α3 | 2.34 |
<τ> f weighed | 2.99 |