Figure 2. NSCs primary culture and Nestin identification.

The neural stem cells were cultured with Neurobasal medium supplemented with 2% B27 and bFGF (20 ng/ml) for 48 h. The halos can be seen clearly around the round-shaped neurospheres (A). The neurospheres showed green fluorescence when they were stained with Nestin (B). Scale bar  = 200 µm.